Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain

The expression of functional proteins on the cell surface using glycosylphosphatidylinositol (GPI)-anchoring technology is a promising approach for constructing yeast cells with special functions. functionality surface-engineered strains strongly depends amount displayed their surface. On other hand, since wall space finite, heterologous protein carrying capacity limited. Here, we report effect CCW12 and CCW14 knockout, which encode major nonenzymatic GPI-anchored (GPI-CWPs) involved in organization, wall. Aspergillus aculeatus β-glucosidase (BGL) was used as reporter to evaluate Saccharomyces cerevisiae. No significant difference wall–associated BGL cell-surface activity observed between knockout control strain. In contrast, co-knockout strains, its were approximately 1.4-fold higher than those strain or strains. Electron microscopic observation revealed that total thickness increased compared parental strain, suggesting potential increase These results indicate are host construction highly recombinant display technology. • and/or BGL-displaying S. cerevisiae knocked out. co-disruption improved efficiency BGL. upon knockout.